human embryonic lung fibroblast cell line mrc 5 (ATCC)

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ATCC human embryonic lung fibroblast cell line mrc 5
Human Embryonic Lung Fibroblast Cell Line Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5462 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+lung+fibroblast+line/MRC-5/pm42271325-65-0-8
Average 99 stars, based on 5462 article reviews

human embryonic lung fibroblast cell line mrc 5 - by Bioz Stars, 2026-10

99/100 stars

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Transfection:

Article Title: Radon Exposure, IL-6 Promoter Variants, and Lung Squamous Cell Carcinoma in Former Uranium Miners
Article Snippet: .. Transfection was performed in a human embryonic kidney cell line and a human lung fibroblast line (HEK293 and HFL1, respectively; both obtained directly from the American Type Culture Collection) using TransIT-2020 transfection reagent (Mirus Bio LLC) and in a normal human bronchial epithelial cell line (HBEC2) immortalized by insertion of the telomerase catalytic subunit and cyclin-dependent kinase 4 (obtained from Shay and Minna, Southwestern Medical Center, Dallas, TX) using a Neon electroporation system (Life Technologies). .. Cells were harvested 48 hr after transfection, and reporter activity was measured using the Dual Luciferase Assay System (Promega).

Electroporation:

other:

Article Title: Carbonate and carbamate modified forms of glucocorticoids in combination with β
Article Snippet: The WI-38 human lung fibroblast line was obtained from the ATCC (catalog number 75-CCL) and maintained in Basal Medium Eagle with Earle's salts (GibcoBRL product number 21010-046) supplemented with 2 mM glutamine and 10% fetal calf serum at 37° C. in a 7% CO2 (balance air), humidified atmosphere.

Article Title: Carbonate and carbamate modified forms of glucocorticoids in combination with B2 adrenergic agonists
Article Snippet: The WI-38 human lung fibroblast line was obtained from the ATCC (catalog number 75-CCL) and maintained in Basal Medium Eagle with Earle's salts (GibcoBRL product number 21010-046) supplemented with 2 mM glutamine and 10% fetal calf serum at 37° C. in a 7% CO2 (balance air), humidified atmosphere.

Cell Culture:

Article Title: Green chemistry approach for the removal of delafloxacin from aqueous solutions using calcinated layered double hydroxide: Adsorption mechanism and material characterization
Article Snippet: .. WI-38 cells, a normal human lung fibroblast line, were obtained from ATCC and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10 % fetal bovine serum (FBS), 1 % L-glutamine, and antibiotics. ..

Modification:

Synthesized:

Article Title: Comprehensive experimental and computational evaluation of novel linagliptin-analogues: Synthesis, DFT calculations, molecular modeling, and in vitro anticancer/antioxidant activities.
Article Snippet: Recent evidence suggests that linagliptin and its structural derivatives exhibit biological activities beyond glycemic control, highlighting the linagliptin scaffold as a promising platform for multifunctional drug design.. In this study, eight novel linagliptin-based azo–imine derivatives (3a–h) were designed, synthesized, and structurally characterized, with the stereochemistry of representative compound 3e confirmed by 2D NMR.. Computational analyses (DFT, molecular docking, and molecular dynamics simulations) were employed to support electronic structure elucidation and ligand–protein interaction stability.

Multiple Displacement Amplification:

Article Title: New bufadienolides extracted from Rhinella marina inhibit Na,K-ATPase and induce apoptosis by activating caspases 3 and 9 in human breast and ovarian cancer cells.
Article Snippet: Bufadienolide compounds have been used for growth inhibition and apoptosis induction in tumor cells.. Those families of cardiotonic steroids can bind the Na,K-ATPase, causing its inhibition.. The use of bufadienolides is widely described in the literature as an anticancer function.

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